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We're seeing these used most often where you need a dead-stop seal at the thread base without relying on tape or sealants — that stepped surface at the root does teh work by extruding the materail slightly as you tighten, which is basically a controlled deformation that fills the micro-gaps, and it works reliably up to about 10 bar depending on polymer you choose.
PP is fine for general lab work at up to 120°C or so, but if you're running aggressive solvents or continuous autoclave cycles, you'll want the PVDF instead — it holds up better chemically, though it's a bit stiffer to handle, and we've had a few buyers switch from Acetal because of hydrolysis issues in high-humidity environments.
The barbed end fits 1.6mm, 3.2mm, or 4.0mm ID tubing pretty snugly, and what it does is it relies on that interference fit rather than a clamp — so you don't need tools, but you do need to push the tubing on firmly, and I'd recommend a quick pressure test before calling it leak-tight.
1/4-28UNF is the most common thread we ship for medical devices, but M5 and M6 are both avaliable — we usually have stock on all three, though lead time can stretch to 20 days or so on the M6 in PA if we're between, so just confirm before ordering.
We run HPLC and include a COA with the lot number, and for medical-grade application our company can supply a material cerification from the resin supplier — it's not something we advertise, but we've had auditors ask for it, so it's there if you need it.
One thing nobody asks but matters: the surface finish is smooth enough that you won't get particulate shedding into a microfluidic channel, but it's not polished to optical clarity — so if you're doing microscopy through the fitting itself, this isn't the part for that.
