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Someone running wound healing assays in a murine model will reconstitute this in sterile water for injection or bacteriostatic water at around 1-2 mg/mL, and they’re typically dosing at 1-2 mg/kg every other day for 10-14 days to see measurable increases in actin polymerization and cell migration — that’s where Thymosin Beta 4 actually shines compared to other angiogenic peptides.
We’ve had labs come back saying they needed tighter purity than the standdard 99%, but honestly, for most in vitro scratch assays or in vivo revascularization studies, that spec holds up fine with minimal batch-to-batch drift (usually well under 0.5% variability by HPLC).
It’s stored at -20°C long-term, but for a 2-week study you can keep it at 4°C without any measurable degradation — though we always recommend aliquoting before freezing because repeated freeze-thaw cycles hammer the carboxyl terminal, and that’s where the G-actin binding activity lives.
The 5mg vials are pretty much teh default choice for single-animal studies, but anyone running on 48-well plates ends up ordering the 10mg vials since you lose about 10% of the powder to vial wall adhesion when you reconstitute below 0.5 mL.
CAS 885340-08-9 is shared by multiple vendors, so what distinguishes batches is endotoxin level — ours typically runs below 0.5 EU/mg, which matters if you’re injecting into immunocompromised mice that don’t tolerate pyrogens well.
Most buyers go with the 15mg vial for multi-target studies, but if you’re only doing endothelial cell scratch assays, the 5mg is actually overkill — you’ll use maybe 2mg total over a week.
We don't have a strict MOQ – you can order as few as 10 vials, even mixed sizes. For larger bulk orders, we can offer better pricing.
Standard shipping takes about 7-15 business days, and we include a tracking number once your order ships. Expedited options are available if you need it sooner.
Yes, every batch comes with a COA showing ≥99% purity by HPLC. Just request it when you place your order, and we'll include it.
We can do custom vial sizes for larger orders, like 50mg or 100mg vials, but it requires a minimum quantity of 100 vials. Reach out to our sales team for details.
For short-term use, keep it refrigerated at 0-4°C. For long-term storage, freeze it at -20°C. Properly stored, it stays stable for over 2 years.
